Day 1 :
Georgia State University, USA
Time : 10:00
Dr. Gabor Patonay is Professor of Analytical Chemistry at Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA. He obtained his M.S. and Ph.D. degrees from the Technical University of Budapest, Hungary. In 1982 he joined Professor Isiah Warner’s group at Emory University, Atlanta, GA, USA. He joined the faculty of GSU in 1987, where he is currently Professor. He spent his years at GSU focusing on NIR fluorescence and developing new bioanalytical and biomedical applications using NIR probes and labels. His recent research interests include bioanalytical and biological applications of fluorescent silica nanoparticles. Dr. Patonay has published over 200 papers. He is the Editor-in-Chief for Analytical Chemistry Insights.
Recent trend for fluorescence detected analytical applications is the use of longer wavelength fluorophores and encapsulation of fluorophores by utilizing nanoparticles. Many applications can be cited such as separations, antibody detection, biomolecule charachterizations, etc. Long wavelength fluorescence in analytical chemistry has several advantages such as lower background interference especially in biological matrices. While many different types of nanoparticles have been utilized for, silica nanoparticles have significant advantages in biological system. Silica nanoparticle synthesis for copolymerization of fluorescent dyes an easily controllable process by using modified TEOS reactive analogues. The fluorescence of silica nanoparticles can significantly be increased by enclosing a large number of dye molecules. For high dye concentrations in the silica nanoparticle self quenching can be significant but it can be minimized by synthesizing large Stokes’ shift dyes or resonance energy transfer. Using NIR dyes excellent sensitivity can be achieved but one major disadvantage is that most biological fluorescence instruments are designed for shorter excitation wavelengths frequently matching the optical properties of fluorescein, a widely used fluorophore. One approach to make these new fluorescent silica nanoparticles more compatible with widely used excitation wavelengths is the development of fluorescent silica nanoparticles containing dyes that are good candidates for fluorescence energy transfer. This presentation discusses the facile synthesis and analytical applications of such silica nanoparticles containing copolymerized multiple fluorophores. The surface properties of fluorescence silica nanoparticles were modified by adding hydrophobic or hydrophilic molecules on the surface to achieve biocompatibility. Biocompatibility was evaluated by hemolytic experiments. Typical applications of these particles are for immunochemistry, flow cytometry, CE, forensic applications, etc. They are also suitable for biomolecule characterization utilizing their specific binding characteristics to biomolecules. Modifying surface properties of silica nanoparticles enhances their analytical utility. Examples will be given for applications of modulated surface hydrophobicity and chirality.
Missouri State University, USA
The Department of Chemistry at Missouri State University-West Plains (MSU-WP) is internationalizing the existing research activities by initiating a collaborative USA-Kenya drug discovery project. Professors Rugutt (MSU-WP) and Kiplimo (University of Kabianga (UoK), Kenya) are indebted to the Carnegie African Diaspora Fellowship (CADF) program for a three-month award that supported the project. Undergraduate, graduate, and high school students played an integral part in implementation of all research and educational activities. The overarching goal of the project is to establish synergistic relationships between different research groups and academic institutions in Kenya and MSU-WP. We chose natural products as the centerpiece of our collaboration because many medicinal Kenyan plants represent an important and underexploited reservoir of potentially new pharmaceutical drugs. Our preliminary results on bioassay-directed fractionation and screening of representative plants afforded several compounds that exhibited various bioactivities including anticancer, antimalarial, anti-HIV, antimicrobial, antimycobaterial, and antifungal. After identifying the most active natural products (sesquiterpene lactones, flavonoids, binaphthyls, steroids, etc.), we conducted a hypothesis-driven green nanosynthetic modifications in order to pinpoint the essential structural features necessary for bioactivity. Novel compounds were prepared based on the world’s best
“named reactions” such as those developed by Nobel Laureates (Diels-Alder, Grubbs, etc.). Our integration of research and education was anchored on the fact that natural products research cuts across many STEM (Science, Technology, Engineering, and Mathematics) disciplines such as medicinal, organic, analytical, and inorganic chemistry. To strengthen preparation of students for STEM careers, chemistry faculty at MSU-WP and UoK are revamping their research and laboratory courses by incorporating current “hot” and inspirational topics in guided-inquiry green chemistry, nanoscience, and natural products. The new courses will provide students with opportunities to learn innovative experimental techniques (chromatography, spectroscopy, bioassays, etc.). Bioassay data from structure-bioactivity relationship studies will be discussed. Also, reports summarizing professional development activities will be disseminated