Jarintzi Yared Rico Ruiz
Euro-Nutec Premix SA de CV, Mexico
Title: An innovative method for the extraction and quantification of curcuminoids from a complex matrix
Biography
Biography: Jarintzi Yared Rico Ruiz
Abstract
In recent years because of the increasing resistance and regulations on the use of antibiotics in animal feed, plant extracts have gained a lot of attention as an effective substitute to antibiotic growth promoters in animal nutrition. Due to the low inclusion levels of essential oils in products, extraction and quantification of the active compounds become a major challenge. As different steps involved in the process can be destructive and affect the properties of natural extracts, its important to have a precise way to quantify the active compounds. In this study a quick and easy method was developed to extract curcuminoids (Bisdemetoxycurcumin-BDMC, Demetoxicurcumin-DMC and Curcumin-CUR) from complex matrix, wich were quantified by Reverse phase Ultra Performance Liquid Cromatography coupled to Mass Spectrometry. For curcuminoids extraction 20g of sample with 60 ml of solvent was deposited in teflon flask, closed and placed in a autoclave for 2 minutes at sterilization temperature, cool down and evaporate with nitrogen until 2 ml remain, filter and analyze. For the 3 curcminoids in premix and feed, the extraction recovery for this method ranged from 94.5 to 99.3 % with relative standard deviation <5% and the detection limit was achieved at levels of micrograms of curcuminoid per gram of sample. Compared to soxhlet extraction method, the method developed offers the advantage of short time and reduced amount of organic solvents used. This metod colud be used to determinate the curcuminoids on complex matrix, wich is needed to evaluate the quality and control of the final products.
