European Chemistry Congress

Biography

Biography: Bonnie Tay-Jones Yen Ping

Abstract

A high-performance liquid-chromatographic (HPLC) method using a diode array detector (DAD) for the determination of low levels of IPTS in cosmetic products has been developed. The optimal conditions for IPTS analysis using this method were found to be as follows: monitoring wavelength of 230 nm; an isocratic mode with a mobile phase of acetonitrile: tetrabutylammonium phosphate monobasic buffer (5 mM); a flow rate of 0.8 ml/min and the use of a reverse phase C8 column. Recoveries of IPTS from cosmetic matrices such as lotions, cleansing milks and creams ranged from 94.0 – 101.1 % with relative standard deviation (RSD) of less than 5 %. A six-point calibration curve (determined over the range 0.5 μg/ml to 50 μg/ml of IPTS) of good linearity with a correlation coefficient of 0.999 was obtained. The limit of detection of the method was found to be 0.5 μg/ml and the limit of quantification was 1.6 μg/ml. The identity of the IPTS recovered from the cosmetic samples was confirmed using a Gas Chromatography-mass spectrometer detector (GC-MSD). This method is thus applicable for analyses of trace levels of IPTS in cosmetic products and has the advantage of using only simple sample preparation steps.