Radovan Buffa
Contipro Pharma, Czech Republic
Title: Conjugates of hyaluronic acid with tyramine synthesis and application
Biography
Biography: Radovan Buffa
Abstract
Hyaluronic acid (HA) is a natural linear polyglycosaminoglycan composed of repetitive disaccharide units, each consisting of N-acetyl-D-glucosamine and D-glucuronic acid. This polysaccharide is an important part of the extra-cellular matrix, vitreous humour and skin. HA modified with a tyramine moiety (HA-tyr) is a biocompatible polymer soluble in water, widely used for many biomedicinal applications. The main advantage of the HA-tyr is its ability to react with various phenolic groups under physiological conditions. This capability has been extensively used mainly for the preparation of biodegradable hydrogels using cross-linking reactions between tyramine molecules. This work presents a simple and effective method for synthesis of HA-Tyr conjugates connected via C6 linker: Tyramine is condensed with 6-aminohexanoic acid and the final substrate is attached on HA-aldehyde by reductive amination. Optimised method is suitable for production in industrial scale.