Maulidiyah
University of Indonesia, Indonesia
Title: Cytotoxic activity of eumitrin A1 from lichen Usnea blepharea motyka
Biography
Biography: Maulidiyah
Abstract
The purpose of this study was to isolate and elucidate structure of secondary metabolite compounds from the lichen Usnea blepharea Motyka that was obtained from Sulawesi – Indonesia, then performed a bioactivity test against Murine Leukemia P388 cells. Powder of lichen has extracted using an acetone solvent and thin layer chromatography (TLC), that continued with separation by using column chromatography (CC) with n-hexane and ethyl acetate solvents in a gradient to produce of pure isolates. The structure was determined by using spectroscopy i.e. UV-Vis, LC-MS, FTIR, 1H-NMR, 13C-NMR, 2D-NMR (HMBC, HMQC, COSY). The results obtained using UV-Vis spectrophotometer gives max wavelength of 229 nm and 334 nm, respectively. From LC-MS data this compound has molecule weight of 680. 1H-NMR, 13C-NMR with DEPT spectrums showed this compound has 34 carbon atoms consisting of 2 methyl groups, 2 methoxy groups, 1 methyl group, 6 metin groups, 3 methylene groups, and 20 C quartener atoms. Supported by using 2D-NMR (HMBC, HMQC, COSY) spectrums the result of analysis can be inferred this compound is a Eumitrin A1 with the formula structure C34H32O15. The result of cytotoxic activity test against Murine Leukemia P388 cells showed very active with IC50 is 4.5 µg/mL.