S Satyanarayana
Osmania University, India
Title: Exploring DNA interaction and anticancer activity of ruthenium (II) mononuclear complexes and their luminescent properties
Biography
Biography: S Satyanarayana
Abstract
A series of mononuclear Ru(II) polypyridyl complexes with N, N-donar ligands (phen = 1, 10 phenanthroline, bpy = 2, 2’ bipyridine, dmb = 4, 4’-dimethyl 2, 2’ bipyridine) and different intercalating ligands (ptip=2-(5-Phenylthiophen-2-yl)-1H-imidazo [4,5-f][1,10] phenanthroline; Bripc = (6-bromo-3-(1H-imidazo[4,5-f] [1,10]-phenanthroline; mipc = 2-(6-methyl-3-(1H-imidazo[4,5-f][1,10]-phenanthroline-2-yl)-4H-chromene-4-one) have been synthesized and characterized by various spectral methods. The binding abilities of ruthenium complexes were investigated using UV-visible and Fluorescence studies. The mode of binding was confirmed by viscosity experiment. Experimental results suggested that they can bind through intercalative mode with DNA having different binding constant. Theoretically, molecular docking studies supported the DNA binding ability of these complexes. These complexes were effectively cleaving the pBR-322 DNA by generating singlet oxygen and encouraging antibacterial activity against Gram-positive and Gram-negative bacterial strains. It was found that cell viability of these complexes shown significant dose dependent cytotoxicity on human cancer cell lines HeLa. The apoptosis assay was carried out with Acridine Orange (AO) staining methods and the results indicate that complexes can induce the apoptosis of HeLa cells. The cell cycle arrest investigated by flow cytometry and these results indicate that all complexes induce the cell cycle arrest at G0/G1phase.
