Yahdiana Harahap
Universitas Indonesia, Indonesia
Title: Quantification of valproic acid in human plasma using high performance liquid chromatography-photodiode array
Biography
Biography: Yahdiana Harahap
Abstract
Valproic acid is one of the mostly used antiepileptic drug that has a lot of side effects, therefore it is highly recommended to determine its plasma concentration. This research objective is to develop an analytical method of valproic acid without derivation in human plasma starting from optimum chromatography condition, optimum plasma preparation method, analysis method validation, until application of validated analysis method. The optimum chromatographic condition was obtained using: C-8 Symmetry (5µm; 150 x 3.9mm), temperature column, the mobile phase contains buffer sodium dihydrogen phosphate 40 mM pH 3.5 – acetonitrile (56:44 %v/v); flow rate was 1.00 mL/min; which was detected by photodiode array detector at wavelength of 210 nm; and nonanoic acid as internal standard. Optimum preparation method is liquid-liquid extraction method using phosphate acid and n-hexana extracted with 150 µL trietylamine 0.5%; shaken with vortex for 2 minutes; and centrifuged for 10 minutes. Validation result of valproat acid analysis method fulfilled the validation requirement using EMEA Bioanalytical Guideline in the year 2011. The acquired method is linear at concentration range of 2.0 – 200.0 µg/mL with r>0.9992. This validated method analysis was successfully applied on one healthy subject to determine valproic acid concentration after oral administration of 500mg valproic acid extended release caplet.
