Ghadeer A. R. Y. Suifan
The University of Jordan, Jordan
Title: Simple colorimetric method for the detection of prostate cancer biomarker prostate specific antigen
Biography
Biography: Ghadeer A. R. Y. Suifan
Abstract
Prostate cancer diagnostic techniques are often complex and require the use of expensive instrumentation. To conquer these limitation, a novel, simple, sensitive and selective colorimetric biosensor for the detection of prostate cancer diagnostic Prodtate Specific Antigen (PSA) biomarker was developed. The biosensing configuration was based on the measurement of PSA. protease proteolytic activity using specific PSA substrate covalently bound to magnetic nanparticles (MNPs) from one terminal and to a gold sensor surface by the other terminal. When intact, the golden sensor appears black by naked eyes. However, upon proteolysis, the cleaved peptide-MNPs moiety will be attracted by an external magnet revealing the sensor surface golden color observable by the naked eye. This biosensor monitor PSA concentration ranging from 1 pg/ mL1 to 1 u/gmL with a detection limit as low as 1 pg/ L in less than 5 minutes. This method demonstrated tremendous sensitivity in addition to specificity to PSA. Moreover, this sensor puts forward a prospective development of wash-less and cost-effective point-of-care device because of the simplicity of the probe immobilization and elimination of the reporter molecule labelling procedure during the biosensing step.