Soulignac Cécile
University of Rouen, France
Title: Surface modification of cyclic olefin copolymer with diazonium salts
Biography
Biography: Soulignac Cécile
Abstract
The modification of metallic surfaces, such as gold, by diazonium salts grafting, has been extently study but modification of polymeric surfaces is still at his premices. Chehimi et al., manage to graft diazonium salt onto polymethylmethacrylate surface in the presence of a reducing reagent (hypophosphorous acid) in 2014 and in our group, this method was adapted to cyclic olefin copolymer (COC) with success. COC has promising properties such as good transparency, high chemical resistance, low cost, high stiffness. Despite these advantages, COC has a chemically inert and hydrophobic surface that renders biomolecule analysis tricky. Controlling surface functionalization is therefore crucial. In this work the functionalization of COC surface with 4-(carboxymethyl)benzenediazonium and with 4-aminobenzenediazonium were done following the previous method and the effect on the COC structure was studied by various analysis methods (DSC, TGA, fluorescent microscopy, mass spectrometry, etc.). The choice of these two salts was to allow peptidic coupling thanks to the carboxy and the amino function available on the surface. Coupling with streptavidin and with biotin were performed and analysed, a gold surface helped us to control the robustness of our experiment. This method offers a versatil tool to develop specifics biosensors over the same functionalized surface. Examples of coupling with isatin and GABA are under study and diazonium with different functions available (in length and termination) synthesis will be investigated.