Harish Kumar
Ch. Devi Lal University, India
Title: Synthesis of Graphene for the fabrication of Electrochemical Biosensor for the Detection of Baccilus cerrus as Biological Warfare Agent
Biography
Biography: Harish Kumar
Abstract
The rapid, unambiguous detection and identification of Biological Warfare Agents (BWAs) with early warning signals for detecting possible biological attack is a major challenge for military, health and other government defence agencies. The current research is focused on development of amperometric biosensors for the detection of biological warfare agent. For this purpose, a carbon based (Graphene) working electrode containing enzyme alkaline phosphatase, petroleum jelly, cellulose acetate and Poly Vinyl Pyrolidone (PVP), Ferrocene, Horseradish peroxidise, aq. KOH was fabricated. A three electrodes based electrochemical biosensor was used for the electrochemical detection of Baccilus cerrus as biological warfare agent in the presence and absence of Fe3O4 nanoparticles. Fe3O4 nanoparticles were synthesized by sol-gel technique and were characterized by UV-visible, FTIR, TEM and XRD techniques. Change in current response and OCP values help in the detection of biological warfare agent in presence and absence of Fe3O4 nanoparticles. Effects of temperature, stirring and Fe3O4 nanoparticles on the BWA have also been investigated. Storage, stability and reusability of electrochemical biosensor was tested. Optimum working conditions of electrochemical biosensor was also tested. Effect of temperature on current response of the biosensor was also investigated. Graphene based electrochemical biosensor helps in easy, quick and efficient detection of biological warfare agents.