Jyoti Korram
Pt Ravishankar Shukla University, India
Title: A sensitive fluorescence sensor for organophosphate pesticides detection by controlling the surface passivation of carbon quantum dot
Biography
Biography: Jyoti Korram
Abstract
The organophosphorus pesticides (OP) induced inhibition of acetylcholinesterase (AChE) was monitored using carbon quantum dots (CQD). Tunable fluorescence CQDs originated from citric acid were synthesized and characterized. The fluorescence emission was quenched by gold nanoparticles (Au NPs) via fluorescence resonance energy transfer (FRET). Thiocholine, produced from acetylthiocholine hydrolysis by the of AChE, could cause the aggregation of Au NPs and the corresponding recovery of FRET-quenched fluorescence emission. The catalytic activity of AChE could be irreversibly inhibited by OPs, thus, the recovery effect was reduced. By evaluating the fluorescence emission intensity of CQDs, a FRET-based sensing platform for OPs determination of paraoxon was established. The sensing platform showed linear relationship with the paraoxon concentrations in the nM range and the limit of detection (LOD) was very low. Real sample study revealed the applicability of this sensing platform. The results show that the OP sensor is promising for applications in food safety and environmental monitoring.