Zhoucheng Wang
Xiamen University, China
Title: Adsorption of BSA on amino-modified silica-coated magnetic MnFe2O4 nanoparticles
Biography
Biography: Zhoucheng Wang
Abstract
Magnetic nanoparticles (MNPs) have drawn a lot of attention because of their unique properties and potential applications such as the immobilization of proteins and enzymes, drug delivery, etc. However, for bio-related applications, the pure magnetic particles might have the problems associated with the formation of large aggregates, alteration of magnetic properties and their toxicity in the biological system. Therefore, it would be necessary to coat a protective layer to ensure their chemical stability and improve their biocompatibility. Silica was considered to be one of the most ideal coating layers for MNPs due to its reliable chemical stability, biocompatibility, and easy functionalization, making them suitable for conjugation with proteins and in vivo applications. Many research have done on the silica-coated magnetic nanoparticles (SMNPs) resent years. Up to now, those studies mainly focused on the magnetite Fe3O4. MnFe2O4 nanoparticles supposed to be a better material for application due to its higher mass magnetisation and magnetic susceptibility. In this work, the superparamagnetic amino-modified silica-coated magnetic MnFe2O4 nanoparticles (AS-MNPs) have been successfully synthesized to adsorb bovine serum albumin (BSA). Comparing with SMNPs, AS-MNPs supposed to facilitate a strong attachment of protein onto its surface due to their additional surface amino groups. The pH and ionic strength effect on the adsorption of BSA were investigated, and isothermal adsorption of BSA on the nanoparticles was carried out by placing AS-MNPs into different batches of BSA solutions at pH 5.1. As a result, a high loading of BSA of 0.159 mg/g can be anchored on the AS-MNPs.