Alexei V Finkelstein
Institute of Protein Research RAS, Russia
Title: Two complementary views on the protein folding puzzle
Biography
Biography: Alexei V Finkelstein
Abstract
The ability of protein chains to fold spontaneously is a long-standing puzzle in molecular biology. Here, we describe physical theories estimating (i) protein folding time, as the time of overcoming the free-energy barrier separating the native (N) and unfolded (U) states of protein chains, and (ii) the volume of conformation space, which is to be scanned to find the most stable chain fold. In the theory of folding rates, a special role is played by the mid-point of thermodynamic (and kinetic) equilibrium between U and N states. Paradoxically, a theoretical estimate of the "U-to-N" folding time is easier to get from consideration of the "N-to-U" unfolding, because it is easier to outline a good unfolding pathway than a good folding pathway, while, after the physical "detailed balance" law applied to the transition mid-point, the "U-to-N" time equals to the "N-to-U" time. The complementary analysis of the volume of conformation space, which is to be scanned going "from U to N" in a search for the most stable fold, performed at the level of formation and assembly of secondary structures, outlines the upper limit of folding times. It is shown that both theories (based on "N-to-U" and "U-to-N" transitions) delineate the observed range of single-domain globular protein folding rates. The first part of this work (study of the "N to U" transitions) has been supported by the grants of the HHMI, RFBR, MCB, and its second part (study of the "U to N" transitions) by the RSF grant 14-24-00157.