Day 1 :
Keynote Forum
Vakhtang Barbakadze
Tbilisi State Medical University, Georgia
Keynote: Biomacromolecule poly[3-(3,4-dihydroxyphenyl)glyceric acid] with potential therapeutic effect
Time : 08:40-09:05
Biography:
V Barbakadze has completed his PhD and DSci in 1978 and 1999 from Zelinsky Instiute of Organic Chemistry, Moscow, Russia and Durmishidze Institute of Biochemistry and Biotechnology, Tbilisi, Georgia, respectively. From 2006, till to date he is the head of laboratory of plant biopolymers at the Tbilisi State Medical University Institute of Pharmacochemistry. In 1996 and 2002, he has been a visiting scientist at Utrecht University (faculty of pharmacy), The Netherlands, by University Scholarship and The Netherlands organization for scientific research (NWO) Scholarship Scientific Program, respectively. He has published more than 73 papers in reputed journals.
Abstract:
The high molecular (>1000 kDa) water-soluble preparations from different species (Symphytum asperum, S. caucasicum, S. officinale, S. grandiflorum and Anchusa italica) of Boraginaceae family were isolated. According to 13C, 1H NMR, 2D heteronuclear 1H/13C SQC spectral data and 1D NOE experiments their main chemical constituent was found to be poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene] or poly[3-(3,4-dihydroxyphenyl)glyceric acid] (p-DGA). The polyoxyethylene chain is the backbone of this polymer molecule and 3,4-dihydroxyphenyl and carboxyl groups are regular substituents at two carbon atoms in the chain. The repeating unit of this regular polymer is 3-(3,4-dihydroxyphenyl)glyceric acid residue. This compound is a first representative of a new class of natural polyethers. Then the racemic monomer 2,3-dihydroxy-3-(3,4-dihydroxyphenyl)propionic acid (DDPPA) and its virtually pure enantiomers (+)-(2R,3S)-2,3-dihydroxy-3-(3,4-dihydroxyphenyl)propionic acid and 2S,3R)-2,3-dihydroxy-3-(3,4-dihydroxyphenyl)propionic acid were synthesized for the first time via Sharpless asymmetric dihydroxylation of trans-caffeic acid derivatives using an osmium catalyst, a stoichiometric oxidant N-methylmorpholine-N-oxide and enantiocomplementary catalysts cinchona alkaloid derivatives (DHQ)2-PHAL and (DHQD)2-PHA as chiral auxiliaries. p-DGA has wide spectrum of biological activity: anticomplementary, antioxidant, anti-inflammatory properties, burn and wound healing effect. p-DGA and DDPPA exerted anti-cancer efficacy in vitro and in vivo against androgen-dependent and –independent human prostate cancer (PCA) cells via targeting androgen receptor, cell cycle arrest and apoptosis without any toxicity, together with a strong decrease in prostate specific antigen level in plasma. However, our results showed that anticancer efficacy of p-DGA is more effective compared to its synthetic monomer. Overall, this study identifies p-DGA as a potent agent against PCA without any toxicity, and supports its clinical application.
Keynote Forum
Zivko Nikolov
Texas A&M University, USA
Keynote: Protein-specific approach to osteopontin purification from Chlamydomonas reinhardtii
Biography:
Dr. Zivko Nikolov received his PhD degree in chemical engineering from the Iowa State University and is currently Dow Professor in Bioprocess Engineering at Texas A&M University. He is Associate Director of the National Center for Therapeutic Manufacturing at Texas A&M.rnBefore joining Texas A&M in 2003, he was Vice President of Bioprocess Development with ProdiGene Inc. Dr. Nikolov has published more than 90 scientific articles and book chapters in the area of bioprocess engineering and protein recovery. He is a recognized expert in bioprocessing of transgenic plants for production of high-value protein products and therapeutic proteins.
Abstract:
Microalgae in recent years have gained interest as a potential protein expression platform. Eukaryotic microalgae - Chlamydomonas reinhardtii, having a GRAS status, exemplifies the potential of this expression system to produce a variety of therapeutic proteins including vaccines and monoclonal antibodies. Our study focuses on osteopontin (OPN), a versatile extracellular structural protein, cancer biomarker, promoter of cell-mediated immunity, and potential therapeutic protein. OPN is an acidic intrinsically disordered extracellular glycoprotein found in many human tissues and body fluids including bone, skin, urine, milk and blood. The chloroplast of C. reinhardtii has the ability to phosphorylate proteins – a crucial posttranslational modification for in-vivo biomineralization and presumably functional integrity of osteopontin. This study describes the development of a purification process leveraging the unique properties of the target recombinant protein. Osteopontin being a highly acidic protein, much like the majority of host protein in C. reinhardtii, combined with the susceptibility to proteolytic degradation makes the purification development both challenging and intriguing. In an attempt to stabilize and partially purify osteopontin in the algal lysate, we screened several extract pretreatment methods. The impact of pretreatments on osteopontin yield, proteolytic stability and removal of host cell impurities will be presented. We also report and compare capture chromatography of OPN on anion exchange, hydroxyapatite, and Fe- and Ga- immobilized metal affinity resins that were selected to leverage osteopontin’s unique biochemical and structural properties.
Keynote Forum
Injae Shin
Yonsei University, Korea
Keynote: Carbohydrate microarrays for study of glycan associated biological events
Time : 09:05-09:30
Biography:
Injae Shin received his PhD in 1995 from University of Minnesota, USA. After his PhD, he moved to University of California, Berkeley, as a Post-doctoral fellow. In 1998, he was appointed as an Assistant Professor of Department of Chemistry at Yonsei University. Since 2010, he is the Director of National Creative Research Initiative Center for Biofunctional Molecules in the field of Chemical Biology. He has published more than 100 papers in reputed journals and has been serving as an Editorial Board Member of Chemical Society Reviews, ChemBioChem and Molecular BioSystems.
Abstract:
Glycans are involved in various physiological processes through interactions with proteins. Importantly, glycan-mediatedrnbiomolecular interactions play key roles in a number of pathological processes. Accordingly, details of glycan-protein interactionsrnprovide deep insights into the understanding of glycan-associated biological events at the molecular level. For rapid analysis ofrnglycan-mediated recognition events, we have constructed carbohydrate microarrays that consist of diverse glycans densely attachedrnto the solid surface in an orderly arrangement. The notable advantage of the carbohydrate microarray-based technology includes thernsimultaneous analysis of many glycan-protein interactions by using a small amount of carbohydrate samples. The prepared microarraysrn were incubated with proteins or cells to probe glycan-protein or glycan-cell interactions. We have showed that glycan microarrays arernvery powerful tools for studies glycan-mediated recognition events in a high-throughput manner. In this presentation, I will discussrnthe recent applications of glycan microarrays for functional glycomics research.
- Track 1: Fundamentals of Organic Chemistry
Track 2: Inorganic Chemistry
Track 3: Analytical Chemistry
Location: Olimpica 1 & 2
Chair
Ionel I Mangalagiu
Al.I.Cuza University of Iasi, Romania
Co-Chair
Maia Merlani
Tbilisi State Medical University, Georgia